Animal Immunization

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No. 99 Haiwang Road, Chengdu, Sichuan, China

+86 400 8677 715

service@nb-biolab.com

Camelid Animal Immunization

We provide end-to-end camelid immunization services using alpacas, llamas, or camels. Our optimized immunization protocols, combined with flexible antigen formats and advanced monitoring methods, ensure robust immune responses and high-quality PBMC samples for nanobody discovery.

Standard Immunization Protocol

Our standard immunization program spans approximately 49 days, designed to achieve strong and sustained immune responses.

Four antigen injections at two-week intervals

Three blood collection timepoints for immune monitoring

PBMC collection for downstream library construction

This approach ensures efficient maturation of antigen-specific VHH responses and supports high-quality nanobody discovery.

Animal Immunization

Figure 1. Animal Immunization and Blood Collection
(a) Alpaca immunization procedure. (b) Blood collection from alpaca. (c) 49-day immunization timeline. Black arrows indicate injection days (Days 0, 14, 28, 42), and droplet icons represent blood collection (Days 0, 21, 35, 49). Day 0 serum is used as a negative control, while samples collected after immunization are used to measure immune response by titer testing.

Supported Antigen Formats

We support a wide range of immunogen formats to address diverse target types:

Recombinant proteins

Peptides

Whole cells

Virus-like particles (VLPs)

LNP-mRNA

Adenoviral or other viral vector-based antigens

Flexible antigen formats enable successful immunization of challenging targets, including membrane proteins and poorly immunogenic molecules.

Customizable Immunization Strategies

Our immunization workflows are fully customizable based on target characteristics and project goals.

Immunization Species	Adjuvant Options
Alpaca Llama Camel	Freund’s adjuvant Proprietary water-soluble adjuvants
Injection Routes	Strategy Optimization
Subcutaneous Intramuscular Nasal administration	We optimize antigen format, dosing schedule, and delivery method to maximize immune response and nanobody diversity.

Immune Response Monitoring

We monitor immune responses throughout the immunization process to ensure successful antibody generation.

ELISA-based serum titer analysis

Flow cytometry (FACS) analysis

VHH-specific detection assays

These methods allow real-time evaluation of antibody development and optimization of immunization strategies.

Deliverables

Clients will receive:

Pre-immune serum

Immune serum samples

Isolated PBMCs for library construction

Serum titer and immune response data

Comprehensive project report

Optional services:

Immunogen design & production

Nanobody library construction and screening

Why Choose Our Camelid Immunization Service

High-Quality, Naïve Animals

All animals are verified to have no prior exposure to target antigens, ensuring clean immune backgrounds and unbiased antibody generation.

Animal Immunization

Figure 2. Our dedicated 40-acre farm houses high-quality alpacas and llamas imported from South America. With expert breeding and veterinary care, we ensure optimal animal health and project confidentiality.

Customized Immunogen Design

We tailor each antigen to your target and application, offering custom design and preparation of mRNA, VLPs, fusion proteins, and more. Every format is optimized to improve immunogenicity, preserve structural integrity, and ensure reliable downstream VHH discovery.

Proprietary Adjuvant Technology

Our camelid-optimized adjuvants enhance immune responses and boosts HcAb titer while maintaining antigen integrity and minimizing toxicity.

Animal Immunization

Figure 3. Serum titers following alpaca immunization with a camelid immune adjuvant and antigen mixture. The left panel shows total IgG titers after four immunizations; the right panel shows VHH titers. The X-axis represents serum dilution, and the Y-axis indicates OD values measured by ELISA.

Advanced Immune Monitoring

Integrated ELISA and FACS-based assays enable precise monitoring of antibody development.

Proprietary VHH Detection Antibody

Our in-house detection antibody allows sensitive and specific quantification of VHH titers without cross-reactivity.

Animal Immunization

Figure 4. Left: Our AlpHcAb detection antibody demonstrates high affinity across diverse VHH sequences. Right: It shows strong specificity for VHHs without binding other alpaca or cross-species IgGs.

Seamless Integration with Discovery Platforms

Direct compatibility with phage display / yeast display library construction and screening workflows.

Choosing the Right Camelid Species for VHH Antibody Generation

Genus	Common Name	Scientific Name	% Heavy-chain Antibodies vs. Total Antibodies	% with Extra Disulfide Bonds	V Region Homology to Human (%)
Camel	Bactrian Camel	Camelus bactrianus	~70%	~80%	65~70%
Lama	Llama	Lama glama	~45%	~10%	>80%
Alpaca	Alpaca	Vicugna pacos	~45%	~10%	>80%

Related Services

Immunogen Design & Production

Phage Display Nanobody Discovery

Yeast Display Nanobody Discovery

Typical Project Timeline

Package	Service Step	Deliverables	Timeline
Optional	Immunogen preparation	Prepared immunogen and QC report	2–6 weeks
Basic	Animal immunization & monitoring	Serum samples, PBMCs, project report	~7 weeks

A standard camelid immunization program requires approximately 7 weeks. If immunogen preparation is included, the total timeline may extend to 9–13 weeks, depending on antigen format and complexity.