Yeast Surface Display Platform

Yeast Surface Display Mechanism

Our YSD platform is based on the α-agglutinin display system. In this system, the antibody fragments or peptides gene sequence is fused to the Aga2p subunit, which is secreted and anchored to the yeast cell wall via disulfide bonds to the Aga1p subunit. Upon induction with galactose, the fusion protein is expressed and displayed on the yeast cell surface, enabling direct interaction with fluorescently labeled antigens during screening. This design allows efficient display of functional VHH molecules for downstream screening and affinity analysis.

Figure 1. The VHH gene and HA tag are inserted into the Aga2 subunit,, and the VHH-Aga2p fusion proteins are anchored to the yeast cell wall via disulfide bonds to Aga1p.

Yeast Display Screening Workflow

Yeast Surface Display Platform

Platform Advantages

Compared with other discovery technologies, yeast display offers several key advantages:

Direct linkage between genotype and phenotype

Eukaryotic expression system enabling proper protein folding

High display density (10⁴–10⁵ copies per cell)

Quantitative and tunable selection using FACS

Multi-parameter screening (affinity, specificity, epitope competition)

Reduced screening bias and lower false-positive rates

Compatibility with both NGS and monoclonal (Sanger-based) analysis

Applications and Molecule Formats

Our YSD Platform supports the display and screening of various biomolecules, including: Nanobodies (VHH), Single-chain antibodies (scFv) ,Fab fragments, Peptides, and other proteins.

Phage Display vs Yeast Display

Feature	Phage Display	Yeast Display
Expression system	Prokaryotic	Eukaryotic
Display copies	Single copy	10⁴–10⁵ copies
Display efficiency	~10%	65–80%
Screening method	Panning	MACS + FACS
False positives	Higher	Lower
Screening throughput	Limited	High with NGS

Both technologies are complementary and can be selected based on project requirements.

Services Powered by This Platform

This platform supports several CRO services:

Yeast Display Nanobody Discovery

Premade Library Screening

Nanobody Affinity Maturation

Yeast Display Peptide Library Screening

Why Choose Our Yeast Display Platform

Robust Technology Platform

Our yeast display system has been optimized for stable and efficient nanobody display.

High-Diversity Libraries

Library sizes exceeding 10⁸ clones provide broad sequence coverage.

High Display Efficiency

Typical display rates reach 70–80%, ensuring strong screening signals.

Advanced FACS Screening

Multi-color flow cytometry enables precise selection based on affinity and specificity.