Phage Display Platform

Phage Display Mechanism

NBbiolab Phage Display platform is based on the M13 filamentous bacteriophage system. In this system, VHH or antibody fragment genes are fused to the gene encoding the phage coat protein pIII. The fusion proteins are displayed on the surface of the phage particles, while the encoding DNA is packaged inside the phage.

In this system, the phenotype (displayed protein) is physically linked to its genotype (encoding DNA), allowing efficient enrichment of target-specific binders through iterative selection and amplification cycles.

Phage Display Library Construction

Our platform ensures high-quality phage display libraries through optimized molecular design and strict quality control.

Zero-Background Cloning

Background-free cloning technology ensures near-complete insertion efficiency and eliminates empty vectors.

Library Diversity

Phage display libraries typically exceed 1 × 10⁹ clones, enabling broad coverage of antibody diversity.

Clonal Uniqueness

Random clone sequencing confirms high diversity with no detectable sequence duplication.

ORF Accuracy

Sequencing validation ensures >90% correct open reading frames.

Phage Display Screening Workflow

Our platform supports flexible screening strategies based on target characteristics.

Solid-Phase Screening

Antigens immobilized on plates or solid surfaces

Widely used for purified protein targets

Liquid-Phase Screening

Antigen binding occurs in solution

Suitable for cell surface proteins or conformational epitopes

Phage Display Platform

Platform Advantages

Key advantages of our phage display platform include:

Direct genotype–phenotype linkage for efficient selection

Ultra-large library capacity for broad diversity coverage

Flexible screening formats adaptable to different target types

Compatibility with a wide range of antigens

Rapid enrichment through iterative panning cycles

Robust and well-established technology

Applications and Molecule Formats

Our phage display platform supports a wide range of applications and molecular formats.

Target Types

Purified proteins

Membrane proteins

Whole cells

Tissue samples

Biotinylated antigens

Molecule Formats

Nanobodies (VHH)

scFv and Fab fragments

Peptides and other protein binders

This versatility makes phage display particularly suitable for early-stage discovery and challenging targets.

Services Powered by This Platform

This platform supports several CRO services:

Phage Display Nanobody Discovery

Premade Library Screening

Why Choose Our Phage Display Platform

Ultra-Large Library Capacity

Libraries exceeding 10⁹ clones provide extensive sequence diversity.

Rapid and Efficient Screening

Optimized workflows enable fast enrichment and identification of binders.

Zero-Background Cloning Technology

Ensures high insertion efficiency and minimizes non-functional clones.

Flexible Screening Strategies

Supports both solid-phase and liquid-phase panning approaches.

Broad Target Compatibility

Applicable to proteins, cells, and complex biological samples.

Proven and Reliable Technology

A mature platform validated across numerous antibody discovery projects.