Nanobody Expression & Production

NBbiolab nanobody expression platform provides flexible production solutions for research, screening, and early therapeutic development. We support multiple expression hosts and molecular formats, enabling rapid transition from sequence to purified material for functional characterization and engineering workflows.

Supported Expression Formats

Monovalent VHH

VHH-Fc fusion proteins

Bispecific/multivalent VHH constructs

Humanized nanobodies

Tagged recombinant proteins

Antibody fragments & engineered variants

Expression Platforms

Mammalian Cell Expression

HEK293/CHO-based transient expression systems for therapeutic-relevant, high-quality proteins, supporting Fc fusions and complex engineered formats. Ideal for functional characterization, cell-based assays, and early therapeutic evaluation.

Microbial Expression

Rapid, cost-effective bacterial (E. coli) system for screening-stage applications. Suitable for early binder validation, high-throughput candidate screening, and non-Fc nanobody production.

Our Core Capabilities

Rapid Expression Workflow

Optimized cloning and transient expression workflows enable efficient progression from sequence to purified protein.

Flexible Construct Engineering

Codon optimization

Tag engineering

Fc fusion formatting

Bispecific construct design

Custom vector systems

Purification & Quality Control (QC)

SDS-PAGE

Purity assessment

Concentration analysis

Optional SEC analysis

Optional endotoxin testing

Scalable Production

Support for small-scale exploratory production and larger-scale preparation for downstream studies.

Expression Workflow

Sequence & construct design

Codon optimization & cloning

Expression host selection

Transient/microbial expression

Protein purification & QC

Optional characterization

Deliverables

Standard Deliverables

Purified recombinant nanobody proteins

Optional expression-ready plasmids

QC analysis & purity assessment reports

Optional Downstream Services

Affinity measurement

Functional characterization

Typical Project Timeline

Stage	Deliverables	Timeline
Construct design & cloning	Expression constructs	2 weeks
Small scale production	Purified nanobody proteins
Quality control	QC analysis report
Optional characterization	Binding/functional assay data	1–2 weeks

FAQs

What expression systems are used for single-domain antibodies?

Both prokaryotic and eukaryotic expression systems are applied for single-domain antibody production. Eukaryotic expression systems are the most commonly used, mainly adopting HEK293 or CHO cells.

What formats are commonly used for expression verification of single-domain antibodies?

The most commonly used format for expression verification is VHH-Fc fusion, with optional Fc subtypes customized per project requirements. VHH-His format can also be used for verification; it is necessary to optimize subsequent activity and functional experimental protocols in advance if this format is adopted.