First Name*

Last Name*

Phone*

E-mail*

Company/institution*

Target Antigen Information

Target antigen origin

Human

Rodent

Canine

Bacterial

Viral

Other (please specify):

Target antigen class

Soluble Protein

Cell Surface Protein

Single-Pass Transmembrane Protein

Multi-Pass Transmembrane Protein

Protein Complex

Intracellular Protein

Other (please specify):

Target antigen molecular weight

Species homology of Target antigen (%) (Cynomolgus monkey, Mouse, Alpaca, Llama, Camel, Other)

Please specify the target protein name for this project

Notice: If you select "No," NBbiolab will follow the customer's project plan and will provide technical guidance only upon request.

No, the target information will remain confidential throughout the project.

Yes, please specify: Target protein name Target protein UniPort ID

Desired Nanobody Properties

Cross-Species Reactivity (please specify):

Epitope-Specific Binding (please specify):

Blocking Activity (please specify):

Antagonistic Activity (please specify):

Agonistic Activity (please specify):

Cell-Surface Binding Activity

High Affinity Binding

Internalization Capability

Other (please specify):

Animal immunization program

Animal species

Alpaca

Llama

Camel

How many

Immunogen type

Protein

Antigen-positive stable cell line

Polypeptide

VLP

LNP-mRNA

Other (please specify):

Immunization details

For the immunization plan, if you have already confirmed the details with NBbiolab, please fill them in directly. If further discussion and confirmation are needed, please let us know, and our technical experts will assist you.

Screening patform

(Leave blank if you would like NBbiolab to recommend an appropriate screening strategy.)

Phage Display Screening + Monoclonal Clone DNA Sequencing

Yeast Surface Display Screening + Monoclonal Clone Sanger Sequencing

Yeast Surface Display Screening + Pooled Clone NGS Analysis

Other (please specify):

Downstream Development Services

(leave blank if consultation with NBbiolab is needed.)

Nanobody expression system

VHH-His Expression (Prokaryotic Expression System)

VHH-hFc Fusion Protein Expression (CHO or HEK293F Mammalian Expression System)

Other (please specify):

Nanobody in vitro functional assay

ELISA assay (EC50/IC50 Determination)

Flow Cytometry (FACS) assay (EC50/IC50 Determination)

Affinity measurement

Antibody Internalization Assay

Other (please specify):

Nanobody Engineering

Humanization

Affinity maturation

Other (please specify):

Antibody Developability Evaluation

Non-Specific Binding Assessment (BSA, HSA, KLH, Insulin, DNA, etc.)

Quality Characterization (SEC, SDS-PAGE, CE-SDS, cIEF, HIC, etc.)

Thermal Stability Assessment (Tm Determination by DSF)

Accelerated Stability Study (37°C for 7 and 14 days, followed by SEC and CE analysis)

Freeze–Thaw Stability Assessment (followed by SEC analysis)

Plasma Stability Study (37°C for 7 and 14 days, followed by ELISA analysis)

Stability Assessment Under Freeze–Thaw and Low-pH Stress Conditions (pH 3.5 for 4 hours), followed by SEC and CE analysis

Other (please specify):

If other, please specify

Any additional information or special requests?